Degradome sequencing
Degradome sequencing (Degradome-Seq),[1][2] also referred to as parallel analysis of RNA ends (PARE),[1][2] is a modified version of 5'-Rapid Amplification of cDNA Ends (RACE) using high-throughput, deep sequencing method using as Illumina's SBS technology. Degradome sequencing provides a comprehensive means of analyzing patterns of RNA degradation.
Degradome sequencing has been used to identify microRNA (miRNA) cleavage sites,[3] because miRNAs can cause endonucleolytic cleavage of mRNA by extensive and often perfect complementarity to mRNAs.[1][2] Degradome sequencing revealed many known and novel plant miRNA (siRNA) targets.[1][2][4][5][6][7] Recently, degradome sequencing also has been applied to identify animal (human and mouse) miRNA-derived cleavages.[8][9][10]
External links
- starBase database: a database for exploring microRNA cleavage sites from degradome sequencing (Degradome-Seq) data.
References
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