PCAF

K(lysine) acetyltransferase 2B
PDB rendering based on 1cm0.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1CM0, 1JM4, 1N72, 1WUG, 1WUM, 1ZS5, 2RNW, 2RNX, 3GG3, 4NSQ
Identifiers
Symbols	KAT2B ; CAF; P/CAF; PCAF
External IDs	OMIM: 602303 MGI: 1343094 HomoloGene: 20834 ChEMBL: 5500 GeneCards: KAT2B Gene
EC number	2.3.1.48
Gene ontology Molecular function • RNA polymerase II regulatory region sequence-specific DNA binding • chromatin binding • transcription cofactor activity • transcription coactivator activity • histone acetyltransferase activity • lysine N-acetyltransferase activity, acting on acetyl phosphate as donor • cyclin-dependent protein serine/threonine kinase inhibitor activity • protein binding • transcription factor binding • acetyltransferase activity • protein kinase binding • protein complex binding • histone acetyltransferase binding • histone deacetylase binding Cellular component • PCAF complex • kinetochore • nuclear chromatin • nucleus • nucleoplasm • Ada2/Gcn5/Ada3 transcription activator complex • cytosol • A band • I band • actomyosin Biological process • chromatin organization • chromatin remodeling • transcription from RNA polymerase I promoter • transcription initiation from RNA polymerase I promoter • transcription initiation from RNA polymerase II promoter • protein acetylation • cell cycle arrest • Notch signaling pathway • memory • negative regulation of cell proliferation • gene expression • regulation of protein ADP-ribosylation • positive regulation of neuron projection development • viral process • N-terminal peptidyl-lysine acetylation • internal peptidyl-lysine acetylation • peptidyl-lysine acetylation • cellular response to insulin stimulus • cellular response to oxidative stress • positive regulation of chromatin binding • positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter • histone H3 acetylation • histone H3-K9 acetylation • negative regulation of cyclin-dependent protein serine/threonine kinase activity • positive regulation of vasodilation • positive regulation of transcription from RNA polymerase II promoter • rhythmic process • cellular response to parathyroid hormone stimulus • positive regulation of histone H3-K14 acetylation • positive regulation of histone H3-K9 acetylation Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	8850	18519
Ensembl	ENSG00000114166	ENSMUSG00000000708
UniProt	Q92831	Q9JHD1
RefSeq (mRNA)	NM_003884	NM_001190846
RefSeq (protein)	NP_003875	NP_001177775
Location (UCSC)	Chr 3: 20.04 – 20.15 Mb	Chr 17: 53.57 – 53.67 Mb
PubMed search	[1]	[2]

P300/CBP-associated factor (PCAF), also known as K(lysine) acetyltransferase 2B (KAT2B), is a human gene and transcriptional coactivator associated with p53.

Structure

Several domains of PCAF can act independently or in unison to enable its functions. PCAF has separate acetyltransferase and E3 ubiquitin ligase domains as well as a bromodomain for interaction with other proteins. PCAF also possesses sites for its own acetylation and ubiquitination.

Function

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by the PCAF gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.

Regulation

The acetyltransferase activity and cellular location of PCAF are regulated through acetylation of PCAF itself. PCAF may be autoacetylated (acetylated by itself) or by p300. Acetylation leads to migration to the nucleus and enhances its acetyltransferase activity. PCAF interacts with and is deacetylated by HDAC3, leading to a reduction in PCAF acetyltransferase activity and cytoplasmic localisation.

Protein interactions

PCAF forms complexes with numerous proteins that guide its activity. For example PCAF is recruited by ATF to acetylate histones and promote transcription of ATF4 target genes.

Targets

There are various protein targets of PCAF's acetyltransferase activity including transcription factors such as Fli1, p53 and numerous histone residues. Hdm2, itself a ubiquitin ligase that targets p53, has also been demonstrated to be a target of the ubiquitin-ligase activity of PCAF.

Interactions

PCAF has been shown to interact with:

• BRCA2,
• CTNNB1,
• CREBBP,
• EVI1,
• HNF1A,
• IRF1,
• IRF2,
• KLF13,
• Mdm2
• Myc,
• NCOA1,
• POLR2A,
• RBPJ,
• TCF3,
• TRRAP, and
• TWIST1.

See also

• Transcription coregulator
• Acetyltransferase

External links

• PCAF protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)
• NURSA C105

Further reading

This article incorporates text from the United States National Library of Medicine, which is in the public domain.